A systematic evaluation of nucleotide properties for CRISPR sgRNA design

A systematic evaluation of nucleotide properties for CRISPR sgRNA design

Abstract Background CRISPR is a versatile gene editing tool which has revolutionized genetic research in the past few years. Optimizing sgRNA design to improve the efficiency of target/DNA cleavage is critical to ensure the success of CRISPR screens. Results By borrowing knowledge from oligonucleoti...

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Bibliographic Details
Main Authors: Pei Fen Kuan, Scott Powers, Shuyao He, Kaiqiao Li, Xiaoyu Zhao, Bo Huang
Format: Article
Language:English
Published: BMC 2017-06-01
Series:BMC Bioinformatics
Subjects:
CRISPR
Machine learning
Predictive modeling
Thermodynamics
Online Access:http://link.springer.com/article/10.1186/s12859-017-1697-6

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http://link.springer.com/article/10.1186/s12859-017-1697-6

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