An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue.

An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue.

A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-mOrange (V2mO) that contained both a puromycin selection and a fluorescent marker, making viral production and target transduction visible. Lentiviruses packaged with this plasmid and appropriate guid...

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Bibliographic Details
Main Authors: Jiankang Jin, Yan Xu, Longfei Huo, Lang Ma, Ailing W Scott, Melissa Pool Pizzi, Yuan Li, Ying Wang, Xiaodan Yao, Shumei Song, Jaffer A Ajani
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0228910

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https://doi.org/10.1371/journal.pone.0228910

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