A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress

A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress

Histone H2AX phosphorylated at serine 139 (γ-H2AX) is a hallmark of DNA damage, signaling the presence of DNA double-strand breaks and global replication stress in mammalian cells. While γ-H2AX can be visualized with antibodies in fixed cells, its detection in living cells was so far not possible. H...

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Bibliographic Details
Main Authors: Eric Moeglin, Dominique Desplancq, Audrey Stoessel, Christian Massute, Jeremy Ranniger, Alastair G. McEwen, Gabrielle Zeder-Lutz, Mustapha Oulad-Abdelghani, Manuela Chiper, Pierre Lafaye, Barbara Di Ventura, Pascal Didier, Arnaud Poterszman, Etienne Weiss
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Cancers
Subjects:
H2AX
phosphorylation
replication stress
nanobody
imaging
one-step detection
Online Access:https://www.mdpi.com/2072-6694/13/13/3317

Internet

https://www.mdpi.com/2072-6694/13/13/3317

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