Purification and cryoelectron microscopy structure determination of human V-ATPase

Purification and cryoelectron microscopy structure determination of human V-ATPase

Summary: Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are multi-component, ATP-driven proton pumps, which play important roles in many physiological processes by acidifying intracellular vesicles, organelles, and the extracellular milieu. Long-standing challenges in purifying ma...

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Bibliographic Details
Main Authors: Longfei Wang, Zhenhang Chen, Hao Wu, Tian-Min Fu
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Biophysics
Cryo-EM
Molecular biology
Protein biochemistry
Protein expression and purification
Structural biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000575

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http://www.sciencedirect.com/science/article/pii/S2666166721000575

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