Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model

Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model

Brown algae are known to contain rich anti-inflammatory compounds. The objective of this study was to identify anti-inflammatory compound in the Sargassum macrocarpum and anti-inflammatory effect of tuberatolide B (TTB) was investigated. TTB significantly suppressed the production of NO and pro-infl...

Full description

Bibliographic Details
Main Authors: Eun-A Kim, Seo-Young Kim, Junseong Kim, Jae-Young Oh, Hyun-Soo Kim, Weon-Jong Yoon, Do-Hyung Kang, Soo-Jin Heo
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:Journal of Functional Foods
Subjects:
Sargassum macrocarpum
Tuberatolide B
Anti-inflammatory
RAW264.7 cells
Zebrafish
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464618305516
id doaj-6210408496234b31bb1b8396e6276bc4
record_format Article
spelling doaj-6210408496234b31bb1b8396e6276bc42021-04-30T07:14:58ZengElsevierJournal of Functional Foods1756-46462019-01-0152109115Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish modelEun-A Kim0Seo-Young Kim1Junseong Kim2Jae-Young Oh3Hyun-Soo Kim4Weon-Jong Yoon5Do-Hyung Kang6Soo-Jin Heo7Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of KoreaJeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of KoreaJeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP), Jeju 63608, Republic of KoreaJeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of Korea; Jeju Department of Marine Biology, University of Science and Technology, Daejeon 34113, Republic of KoreaJeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of Korea; Jeju Department of Marine Biology, University of Science and Technology, Daejeon 34113, Republic of Korea; Corresponding author at: Jeju International Marine Science Center for Research Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of Korea.Brown algae are known to contain rich anti-inflammatory compounds. The objective of this study was to identify anti-inflammatory compound in the Sargassum macrocarpum and anti-inflammatory effect of tuberatolide B (TTB) was investigated. TTB significantly suppressed the production of NO and pro-inflammatory cytokines such as interleukin (IL)-6 and IL-1β. In addition, the mechanism involved in the inhibition of the inflammatory effect of LPS by TTB was investigated. The results showed that TTB aforementioned this effect through NF-κB and MAPKs phosphorylation pathways. We also assessed the anti-inflammatory activity of TTB using in vivo zebrafish model. In LPS-stimulated zebrafish, TTB enhanced survival and significantly inhibited the NO production and mRNA expression of inducible NO synthase. Therefore, anti-inflammatory activity of TTB against LPS in RAW264.7 cells and the zebrafish model was determined. These finding suggest that TTB may be used as functional anti-inflammatory foods and nutraceuticals.http://www.sciencedirect.com/science/article/pii/S1756464618305516Sargassum macrocarpumTuberatolide BAnti-inflammatoryRAW264.7 cellsZebrafish
collection DOAJ
language English
format Article
sources DOAJ
author Eun-A Kim
Seo-Young Kim
Junseong Kim
Jae-Young Oh
Hyun-Soo Kim
Weon-Jong Yoon
Do-Hyung Kang
Soo-Jin Heo
spellingShingle Eun-A Kim
Seo-Young Kim
Junseong Kim
Jae-Young Oh
Hyun-Soo Kim
Weon-Jong Yoon
Do-Hyung Kang
Soo-Jin Heo
Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model
Journal of Functional Foods
Sargassum macrocarpum
Tuberatolide B
Anti-inflammatory
RAW264.7 cells
Zebrafish
author_facet Eun-A Kim
Seo-Young Kim
Junseong Kim
Jae-Young Oh
Hyun-Soo Kim
Weon-Jong Yoon
Do-Hyung Kang
Soo-Jin Heo
author_sort Eun-A Kim
title Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model
title_short Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model
title_full Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model
title_fullStr Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model
title_full_unstemmed Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264.7 cells and zebrafish model
title_sort tuberatolide b isolated from sargassum macrocarpum inhibited lps-stimulated inflammatory response via mapks and nf-κb signaling pathway in raw264.7 cells and zebrafish model
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2019-01-01
description Brown algae are known to contain rich anti-inflammatory compounds. The objective of this study was to identify anti-inflammatory compound in the Sargassum macrocarpum and anti-inflammatory effect of tuberatolide B (TTB) was investigated. TTB significantly suppressed the production of NO and pro-inflammatory cytokines such as interleukin (IL)-6 and IL-1β. In addition, the mechanism involved in the inhibition of the inflammatory effect of LPS by TTB was investigated. The results showed that TTB aforementioned this effect through NF-κB and MAPKs phosphorylation pathways. We also assessed the anti-inflammatory activity of TTB using in vivo zebrafish model. In LPS-stimulated zebrafish, TTB enhanced survival and significantly inhibited the NO production and mRNA expression of inducible NO synthase. Therefore, anti-inflammatory activity of TTB against LPS in RAW264.7 cells and the zebrafish model was determined. These finding suggest that TTB may be used as functional anti-inflammatory foods and nutraceuticals.
topic Sargassum macrocarpum
Tuberatolide B
Anti-inflammatory
RAW264.7 cells
Zebrafish
url http://www.sciencedirect.com/science/article/pii/S1756464618305516
work_keys_str_mv AT eunakim tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
AT seoyoungkim tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
AT junseongkim tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
AT jaeyoungoh tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
AT hyunsookim tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
AT weonjongyoon tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
AT dohyungkang tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
AT soojinheo tuberatolidebisolatedfromsargassummacrocarpuminhibitedlpsstimulatedinflammatoryresponseviamapksandnfkbsignalingpathwayinraw2647cellsandzebrafishmodel
_version_ 1721499200868319232

Similar Items