Conditions for gene disruption by homologous recombination of exogenous DNA into the Sulfolobus solfataricus genome
The construction of directed gene deletion mutants is an essential tool in molecular biology that allows functional studies on the role of genes in their natural environment. For hyperthermophilic archaea, it has been difficult to obtain a reliable system to construct such mutants. However, during t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2008-01-01
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| Series: | Archaea |
| Online Access: | http://dx.doi.org/10.1155/2008/948014 |