Molecular cloning, expression, and immobilization of glutamate decarboxylase from Lactobacillus fermentum YS2

Molecular cloning, expression, and immobilization of glutamate decarboxylase from Lactobacillus fermentum YS2

Background: GABA (γ-aminobutyric acid) is a four-carbon nonprotein amino acid that has hypotensive, diuretic, and tranquilizing properties. Glutamate decarboxylase (GAD) is the key enzyme to generate GABA. A simple and economical method of preparing and immobilizing GAD would be helpful for GABA pro...

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Bibliographic Details
Main Authors: Qian Lin, Dongni Li, Huizhen Qin
Format: Article
Language:English
Published: Elsevier 2017-05-01
Series:Electronic Journal of Biotechnology
Subjects:
Adsorbent
Food biotechnology
Fusion protein
GABA production
GABA synthesis
GAD
Microbial biotechnology
Regenerated amorphous cellulose
Stress-inducible promoter
T7 terminator
γ-Aminobutyric acid
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345817300106

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http://www.sciencedirect.com/science/article/pii/S0717345817300106

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