Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.

Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.

CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function screens of thousands of regulatory elements....

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Bibliographic Details
Main Authors: Carlos Pulido-Quetglas, Estel Aparicio-Prat, Carme Arnan, Taisia Polidori, Toni Hermoso, Emilio Palumbo, Julia Ponomarenko, Roderic Guigo, Rory Johnson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-03-01
Series:PLoS Computational Biology
Online Access:http://europepmc.org/articles/PMC5333799?pdf=render

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http://europepmc.org/articles/PMC5333799?pdf=render

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