Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

Abstract Background Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single cells have been proposed as have sin...

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Bibliographic Details
Main Authors: Elena Denisenko, Belinda B. Guo, Matthew Jones, Rui Hou, Leanne de Kock, Timo Lassmann, Daniel Poppe, Olivier Clément, Rebecca K. Simmons, Ryan Lister, Alistair R. R. Forrest
Format: Article
Language:English
Published: BMC 2020-06-01
Series:Genome Biology
Subjects:
Single-cell transcriptomics
RNA-seq
scRNA-seq
snRNA-seq
10x Genomics
Online Access:http://link.springer.com/article/10.1186/s13059-020-02048-6

Internet

http://link.springer.com/article/10.1186/s13059-020-02048-6

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