Optimization of Polycistronic Anti-CCR5 Artificial microRNA Leads to Improved Accuracy of Its Lentiviral Vector Transfer and More Potent Inhibition of HIV-1 in CD4+ T-Cells

Optimization of Polycistronic Anti-CCR5 Artificial microRNA Leads to Improved Accuracy of Its Lentiviral Vector Transfer and More Potent Inhibition of HIV-1 in CD4+ T-Cells

C-C chemokine receptor type 5 (CCR5) is utilized by human immunodeficiency virus (HIV) as a co-receptor for cell entry. Suppression of the CCR5 gene by artificial microRNAs (amiRNAs) could confer cell resistance. In previous work, we created a lentivector that encoded the polycistron of two identica...

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Bibliographic Details
Main Authors: Felix Urusov, Dina Glazkova, Denis Omelchenko, Elena Bogoslovskaya, Galina Tsyganova, Katerina Kersting, German Shipulin, Vadim Pokrovsky
Format: Article
Language:English
Published: MDPI AG 2018-02-01
Series:Cells
Subjects:
HIV gene therapy
CCR5 co-receptor
RNA interference
miRNA
lentiviral vectors
Online Access:http://www.mdpi.com/2073-4409/7/2/10

Internet

http://www.mdpi.com/2073-4409/7/2/10

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