Optimization of Polycistronic Anti-CCR5 Artificial microRNA Leads to Improved Accuracy of Its Lentiviral Vector Transfer and More Potent Inhibition of HIV-1 in CD4+ T-Cells
C-C chemokine receptor type 5 (CCR5) is utilized by human immunodeficiency virus (HIV) as a co-receptor for cell entry. Suppression of the CCR5 gene by artificial microRNAs (amiRNAs) could confer cell resistance. In previous work, we created a lentivector that encoded the polycistron of two identica...
Main Authors: | Felix Urusov, Dina Glazkova, Denis Omelchenko, Elena Bogoslovskaya, Galina Tsyganova, Katerina Kersting, German Shipulin, Vadim Pokrovsky |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-02-01
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Series: | Cells |
Subjects: | |
Online Access: | http://www.mdpi.com/2073-4409/7/2/10 |
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