A Toolbox for Site-Specific Labeling of RecQ Helicase With a Single Fluorophore Used in the Single-Molecule Assay

A Toolbox for Site-Specific Labeling of RecQ Helicase With a Single Fluorophore Used in the Single-Molecule Assay

Fluorescently labeled proteins can improve the detection sensitivity and have been widely used in a variety of biological measurements. In single-molecule assays, site-specific labeling of proteins enables the visualization of molecular interactions, conformational changes in proteins, and enzymatic...

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Bibliographic Details
Main Authors: Fang-Yuan Teng, Zong-Zhe Jiang, Ling-Yun Huang, Man Guo, Feng Chen, Xi-Miao Hou, Xu-Guang Xi, Yong Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-09-01
Series:Frontiers in Molecular Biosciences
Subjects:
fluorescence
molecular interaction
molecular dynamic
DNA repair
single molecule
helicase
Online Access:https://www.frontiersin.org/article/10.3389/fmolb.2020.586450/full

Internet

https://www.frontiersin.org/article/10.3389/fmolb.2020.586450/full

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