FIB-SEM as a Volume Electron Microscopy Approach to Study Cellular Architectures in SARS-CoV-2 and Other Viral Infections: A Practical Primer for a Virologist

FIB-SEM as a Volume Electron Microscopy Approach to Study Cellular Architectures in SARS-CoV-2 and Other Viral Infections: A Practical Primer for a Virologist

The visualization of cellular ultrastructure over a wide range of volumes is becoming possible by increasingly powerful techniques grouped under the rubric “volume electron microscopy” or volume EM (vEM). Focused ion beam scanning electron microscopy (FIB-SEM) occupies a “Goldilocks zone” in vEM: it...

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Bibliographic Details
Main Authors: Valentina Baena, Ryan Conrad, Patrick Friday, Ella Fitzgerald, Taeeun Kim, John Bernbaum, Heather Berensmann, Adam Harned, Kunio Nagashima, Kedar Narayan
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Viruses
Subjects:
FIB-SEM
volumeEM
vEM
SARS-CoV-2
virological synapse
segmentation
Online Access:https://www.mdpi.com/1999-4915/13/4/611

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https://www.mdpi.com/1999-4915/13/4/611

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