Simultaneous Detection of Antigen-Specific IgG- and IgM-Secreting Cells with a B Cell Fluorospot Assay

The traditional enzyme-linked immunospot (ELISpot) assay is the gold standard for the enumeration of antigen-specific B cells. Since B cell availability from biological samples is often limited, either because of sample size/volume or the need of performing multiple analyses on the same sample, the...

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Bibliographic Details
Main Authors: M. Anthony Moody, Mattia Bonsignori
Format: Article
Language:English
Published: MDPI AG 2012-03-01
Series:Cells
Subjects:
Online Access:http://www.mdpi.com/2073-4409/1/1/15