Simultaneous Detection of Antigen-Specific IgG- and IgM-Secreting Cells with a B Cell Fluorospot Assay
The traditional enzyme-linked immunospot (ELISpot) assay is the gold standard for the enumeration of antigen-specific B cells. Since B cell availability from biological samples is often limited, either because of sample size/volume or the need of performing multiple analyses on the same sample, the...
Main Authors: | M. Anthony Moody, Mattia Bonsignori |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-03-01
|
Series: | Cells |
Subjects: | |
Online Access: | http://www.mdpi.com/2073-4409/1/1/15 |
Similar Items
-
Comparison of ELISpot and FluoroSpot in the Analysis of Swine Flu-Specific IgG and IgA Secretion by in Vivo Activated Human B Cells
by: Bernt Axelsson, et al.
Published: (2012-04-01) -
Analyse de réponses lymphocytaires T par Elispot et Fluorospot au cours du suivi de protocoles de vaccination : mise en évidence de l’effet du thiomersal présent dans la préparation vaccinale
by: Chauvat, Anne
Published: (2012) -
P04-01. Simultaneous enumeration of HIV-1 gp41 Env-specific IgG and IgM antibody-secreting cells with a multiplex B-cell fluorospot assay
by: Haynes BF, et al.
Published: (2009-10-01) -
Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays
by: Sylvia Janetzki, et al.
Published: (2014-11-01) -
Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays
by: Sebastian Köppert, et al.
Published: (2021-07-01)