The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T Cells
Short-chain fatty acids (SCFAs), which are generated by the bacterial fermentation of dietary fibers, promote expansion of regulatory T cells (Tregs). Potential therapeutic value of SCFAs has been recently highlighted in the experimental models of T cell-mediated autoimmunity and allergic inflammati...
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doaj-62f3e1da620a48c297344abc7890e0662020-11-24T23:45:21ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-08-01810.3389/fimmu.2017.01036286173The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T CellsMeike Kespohl0Niyati Vachharajani1Maik Luu2Hani Harb3Sabine Pautz4Svenja Wolff5Nina Sillner6Nina Sillner7Alesia Walker8Philippe Schmitt-Kopplin9Philippe Schmitt-Kopplin10Philippe Schmitt-Kopplin11Thomas Boettger12Harald Renz13Stefan Offermanns14Ulrich Steinhoff15Alexander Visekruna16Institute for Medical Microbiology and Hygiene, Philipps University of Marburg, Marburg, GermanyInstitute for Medical Microbiology and Hygiene, Philipps University of Marburg, Marburg, GermanyInstitute for Medical Microbiology and Hygiene, Philipps University of Marburg, Marburg, GermanyInstitute of Laboratory Medicine and Pathobiochemistry, Molecular Diagnostics, Philipps University of Marburg, Marburg, GermanyInstitute for Medical Microbiology and Hygiene, Philipps University of Marburg, Marburg, GermanyInstitute for Medical Microbiology and Hygiene, Philipps University of Marburg, Marburg, GermanyResearch Unit Analytical BioGeoChemistry, Department of Environmental Sciences, Helmholtz Zentrum München, Neuherberg, GermanyZIEL – Institute for Food and Health, Technical University of Munich, Freising, GermanyResearch Unit Analytical BioGeoChemistry, Department of Environmental Sciences, Helmholtz Zentrum München, Neuherberg, GermanyResearch Unit Analytical BioGeoChemistry, Department of Environmental Sciences, Helmholtz Zentrum München, Neuherberg, GermanyZIEL – Institute for Food and Health, Technical University of Munich, Freising, GermanyAnalytical Food Chemistry, Technical University of Munich, Freising, GermanyDepartment of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, GermanyInstitute of Laboratory Medicine and Pathobiochemistry, Molecular Diagnostics, Philipps University of Marburg, Marburg, GermanyDepartment of Pharmacology, Max Planck Institute for Heart and Lung Research, Bad Nauheim, GermanyInstitute for Medical Microbiology and Hygiene, Philipps University of Marburg, Marburg, GermanyInstitute for Medical Microbiology and Hygiene, Philipps University of Marburg, Marburg, GermanyShort-chain fatty acids (SCFAs), which are generated by the bacterial fermentation of dietary fibers, promote expansion of regulatory T cells (Tregs). Potential therapeutic value of SCFAs has been recently highlighted in the experimental models of T cell-mediated autoimmunity and allergic inflammation. These studies suggest that physiological intestinal concentrations of SCFAs within the millimolar range are crucial for dampening inflammation-mediated processes. Here, we describe opposing effects of SCFAs on T cell-mediated immune responses. In accordance with published data, lower butyrate concentrations facilitated differentiation of Tregs in vitro and in vivo under steady-state conditions. In contrast, higher concentrations of butyrate induced expression of the transcription factor T-bet in all investigated T cell subsets resulting in IFN-γ-producing Tregs or conventional T cells. This effect was mediated by the inhibition of histone deacetylase activity and was independent of SCFA-receptors FFA2 and FFA3 as well as of Na+-coupled SCFA transporter Slc5a8. Importantly, while butyrate was not able to induce the generation of Tregs in the absence of TGF-β1, the expression of T-bet and IFN-γ was triggered upon stimulation of CD4+ T cells with this SCFA alone. Moreover, the treatment of germ-free mice with butyrate enhanced the expression of T-bet and IFN-γ during acute colitis. Our data reveal that, depending on its concentration and immunological milieu, butyrate may exert either beneficial or detrimental effects on the mucosal immune system.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01036/fullshort-chain fatty acidsbutyrateregulatory T cellsinterferon-gammainhibition of histone deacetylase activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meike Kespohl Niyati Vachharajani Maik Luu Hani Harb Sabine Pautz Svenja Wolff Nina Sillner Nina Sillner Alesia Walker Philippe Schmitt-Kopplin Philippe Schmitt-Kopplin Philippe Schmitt-Kopplin Thomas Boettger Harald Renz Stefan Offermanns Ulrich Steinhoff Alexander Visekruna |
spellingShingle |
Meike Kespohl Niyati Vachharajani Maik Luu Hani Harb Sabine Pautz Svenja Wolff Nina Sillner Nina Sillner Alesia Walker Philippe Schmitt-Kopplin Philippe Schmitt-Kopplin Philippe Schmitt-Kopplin Thomas Boettger Harald Renz Stefan Offermanns Ulrich Steinhoff Alexander Visekruna The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T Cells Frontiers in Immunology short-chain fatty acids butyrate regulatory T cells interferon-gamma inhibition of histone deacetylase activity |
author_facet |
Meike Kespohl Niyati Vachharajani Maik Luu Hani Harb Sabine Pautz Svenja Wolff Nina Sillner Nina Sillner Alesia Walker Philippe Schmitt-Kopplin Philippe Schmitt-Kopplin Philippe Schmitt-Kopplin Thomas Boettger Harald Renz Stefan Offermanns Ulrich Steinhoff Alexander Visekruna |
author_sort |
Meike Kespohl |
title |
The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T Cells |
title_short |
The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T Cells |
title_full |
The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T Cells |
title_fullStr |
The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T Cells |
title_full_unstemmed |
The Microbial Metabolite Butyrate Induces Expression of Th1-Associated Factors in CD4+ T Cells |
title_sort |
microbial metabolite butyrate induces expression of th1-associated factors in cd4+ t cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-08-01 |
description |
Short-chain fatty acids (SCFAs), which are generated by the bacterial fermentation of dietary fibers, promote expansion of regulatory T cells (Tregs). Potential therapeutic value of SCFAs has been recently highlighted in the experimental models of T cell-mediated autoimmunity and allergic inflammation. These studies suggest that physiological intestinal concentrations of SCFAs within the millimolar range are crucial for dampening inflammation-mediated processes. Here, we describe opposing effects of SCFAs on T cell-mediated immune responses. In accordance with published data, lower butyrate concentrations facilitated differentiation of Tregs in vitro and in vivo under steady-state conditions. In contrast, higher concentrations of butyrate induced expression of the transcription factor T-bet in all investigated T cell subsets resulting in IFN-γ-producing Tregs or conventional T cells. This effect was mediated by the inhibition of histone deacetylase activity and was independent of SCFA-receptors FFA2 and FFA3 as well as of Na+-coupled SCFA transporter Slc5a8. Importantly, while butyrate was not able to induce the generation of Tregs in the absence of TGF-β1, the expression of T-bet and IFN-γ was triggered upon stimulation of CD4+ T cells with this SCFA alone. Moreover, the treatment of germ-free mice with butyrate enhanced the expression of T-bet and IFN-γ during acute colitis. Our data reveal that, depending on its concentration and immunological milieu, butyrate may exert either beneficial or detrimental effects on the mucosal immune system. |
topic |
short-chain fatty acids butyrate regulatory T cells interferon-gamma inhibition of histone deacetylase activity |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.01036/full |
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