A novel immunofluorescence detection method for renal cell-type specific in situ cytokine production by confocal microscopy

The detection of cytokines production in tissues is subjected to significant limitations: (1) Cytokine protein production frequently does not correlate with mRNA levels. (2) Cytokines are secreted rapidly and dissipate from the cellular source, thus making detection difficult. (3) The synthetic rate...

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Bibliographic Details
Main Authors: Sun-sang J. Sung, Shu Man Fu
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016120301552