A novel immunofluorescence detection method for renal cell-type specific in situ cytokine production by confocal microscopy
The detection of cytokines production in tissues is subjected to significant limitations: (1) Cytokine protein production frequently does not correlate with mRNA levels. (2) Cytokines are secreted rapidly and dissipate from the cellular source, thus making detection difficult. (3) The synthetic rate...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-01-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120301552 |