CRISPR Gene Perturbations Provide Insights for Improving Bacterial Biofuel Tolerance

CRISPR Gene Perturbations Provide Insights for Improving Bacterial Biofuel Tolerance

Economically-viable biofuel production is often limited by low levels of microbial tolerance to high biofuel concentrations. Here we demonstrate the first application of deactivated CRISPR perturbations of gene expression to improve Escherichia coli biofuel tolerance. We construct a library of 31 un...

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Bibliographic Details
Main Authors: Peter B. Otoupal, Anushree Chatterjee
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-09-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
dCas9
gene expression
biofuels
tolerance
n-butanol
n-hexane
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2018.00122/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2018.00122/full

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