An optimized approach to germ-free rearing in the jewel wasp Nasonia

Development of a Nasonia in vitrogerm-free rearing system in 2012 enabled investigation of Nasonia-microbiota interactions and real-time visualization of parasitoid metamorphosis. However, the use of antibiotics, bleach, and fetal bovine serum introduced artifacts relative to conventional rearing of...

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Bibliographic Details
Main Authors: J. Dylan Shropshire, Edward J. van Opstal, Seth R. Bordenstein
Format: Article
Language:English
Published: PeerJ Inc. 2016-08-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/2316.pdf