Direct Nanopore Sequencing of mRNA Reveals Landscape of Transcript Isoforms in Apicomplexan Parasites

We have used a novel nanopore sequencing technology to directly analyze parasite transcriptomes. The very long reads of this technology reveal the full-length genes of the parasites that cause malaria and toxoplasmosis. Gene transcripts must be processed in a process called splicing befor...

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Bibliographic Details
Main Authors: V. Vern Lee, Louise M. Judd, Aaron R. Jex, Kathryn E. Holt, Christopher J. Tonkin, Stuart A. Ralph
Format: Article
Language:English
Published: American Society for Microbiology 2021-04-01
Series:mSystems
Online Access:https://journals.asm.org/doi/10.1128/mSystems.01081-20