Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice

Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice

Improved genome-editing via oviductal nucleic acid delivery (i-GONAD) is a technique capable of inducing genomic changes in preimplantation embryos (zygotes) present within the oviduct of a pregnant female. i-GONAD involves intraoviductal injection of a solution containing genome-editing components...

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Bibliographic Details
Main Authors: Masahiro Sato, Rico Miyagasako, Shuji Takabayashi, Masato Ohtsuka, Izuho Hatada, Takuro Horii
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Cells
Subjects:
i-gonad
knock-in
indels
genome-editing
oviducts
electroporation
cre/loxp
in vivo gene delivery
Online Access:https://www.mdpi.com/2073-4409/9/3/546

Internet

https://www.mdpi.com/2073-4409/9/3/546

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