Protocol to profile the bioenergetics of organoids using Seahorse

Summary: Addressing bioenergetics is key to evaluate the impact of metabolism on the regulation of biological processes and its alteration in disease. Organoids are in vitro grown self-organizing structures derived from healthy and diseased tissue that recapitulate with high fidelity the tissue of o...

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Bibliographic Details
Main Authors: Marlies C. Ludikhuize, Maaike Meerlo, Boudewijn M.T. Burgering, Maria J. Rodríguez Colman
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000939