A Cautionary Report for Pathogen Identification Using Shotgun Metagenomics; A Comparison to Aerobic Culture and Polymerase Chain Reaction for Salmonella enterica Identification

A Cautionary Report for Pathogen Identification Using Shotgun Metagenomics; A Comparison to Aerobic Culture and Polymerase Chain Reaction for Salmonella enterica Identification

This study was conducted to compare aerobic culture, polymerase chain reaction (PCR), lateral flow immunoassay (LFI), and shotgun metagenomics for identification of Salmonella enterica in feces collected from feedlot cattle. Samples were analyzed in parallel using all four tests. Results from aerobi...

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Bibliographic Details
Main Authors: Enrique Doster, Pablo Rovira, Noelle R. Noyes, Brandy A. Burgess, Xiang Yang, Margaret D. Weinroth, Lyndsey Linke, Roberta Magnuson, Christina Boucher, Keith E. Belk, Paul S. Morley
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-11-01
Series:Frontiers in Microbiology
Subjects:
shotgun metagenomics
Salmonella enterica
culture
PCR
pathogen identification
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.02499/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.02499/full

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