A universal mariner transposon system for forward genetic studies in the genus Clostridium.

DNA transposons represent an essential tool in the armoury of the molecular microbiologist. We previously developed a catP-based mini transposon system for Clostridium difficile in which the expression of the transposase gene was dependent on a sigma factor unique to C. difficile, TcdR. Here we have...

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Bibliographic Details
Main Authors: Ying Zhang, Alexander Grosse-Honebrink, Nigel P Minton
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4383383?pdf=render