Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed...

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Main Authors: Olga A. Krasheninina, Evgeny K. Apartsin, Elena Fuentes, Aleksandra Szulc, Maksim Ionov, Alya G. Venyaminova, Dzmitry Shcharbin, F. Javier de la Mata, Maria Bryszewska, Rafael Gόmez
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Pharmaceutics
Subjects:
carbosilane
dendrimers
anticancer siRNA
nucleic acids
dendriplexes
complexation
transfection
cytotoxicity
biophysical assays
Online Access:http://www.mdpi.com/1999-4923/11/1/25
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spelling doaj-6479b0ad8ff34feebcaed11a609cd6142020-11-25T02:48:14ZengMDPI AGPharmaceutics1999-49232019-01-011112510.3390/pharmaceutics11010025pharmaceutics11010025Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer CellsOlga A. Krasheninina0Evgeny K. Apartsin1Elena Fuentes2Aleksandra Szulc3Maksim Ionov4Alya G. Venyaminova5Dzmitry Shcharbin6F. Javier de la Mata7Maria Bryszewska8Rafael Gόmez9Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaDepartamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, SpainDepartment of General Biophysics, University of Lodz, 90-236 Lodz, PolandDepartment of General Biophysics, University of Lodz, 90-236 Lodz, PolandInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Biophysics and Cell Engineering of NASB, 220072 Minsk, BelarusDepartamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, SpainDepartment of General Biophysics, University of Lodz, 90-236 Lodz, PolandDepartamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, SpainThis paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.http://www.mdpi.com/1999-4923/11/1/25carbosilanedendrimersanticancer siRNAnucleic acidsdendriplexescomplexationtransfectioncytotoxicitybiophysical assays
collection DOAJ
language English
format Article
sources DOAJ
author Olga A. Krasheninina
Evgeny K. Apartsin
Elena Fuentes
Aleksandra Szulc
Maksim Ionov
Alya G. Venyaminova
Dzmitry Shcharbin
F. Javier de la Mata
Maria Bryszewska
Rafael Gόmez
spellingShingle Olga A. Krasheninina
Evgeny K. Apartsin
Elena Fuentes
Aleksandra Szulc
Maksim Ionov
Alya G. Venyaminova
Dzmitry Shcharbin
F. Javier de la Mata
Maria Bryszewska
Rafael Gόmez
Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
Pharmaceutics
carbosilane
dendrimers
anticancer siRNA
nucleic acids
dendriplexes
complexation
transfection
cytotoxicity
biophysical assays
author_facet Olga A. Krasheninina
Evgeny K. Apartsin
Elena Fuentes
Aleksandra Szulc
Maksim Ionov
Alya G. Venyaminova
Dzmitry Shcharbin
F. Javier de la Mata
Maria Bryszewska
Rafael Gόmez
author_sort Olga A. Krasheninina
title Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_short Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_full Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_fullStr Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_full_unstemmed Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_sort complexes of pro-apoptotic sirnas and carbosilane dendrimers: formation and effect on cancer cells
publisher MDPI AG
series Pharmaceutics
issn 1999-4923
publishDate 2019-01-01
description This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.
topic carbosilane
dendrimers
anticancer siRNA
nucleic acids
dendriplexes
complexation
transfection
cytotoxicity
biophysical assays
url http://www.mdpi.com/1999-4923/11/1/25
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