Long-Range PCR-Based NGS Applications to Diagnose Mendelian Retinal Diseases

Long-Range PCR-Based NGS Applications to Diagnose Mendelian Retinal Diseases

The purpose of this study was to develop a flexible, cost-efficient, next-generation sequencing (NGS) protocol for genetic testing. Long-range polymerase chain reaction (PCR) amplicons of up to 20 kb in size were designed to amplify entire genomic regions for a panel (<i>n </i>= 35) of i...

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Bibliographic Details
Main Authors: Jordi Maggi, Samuel Koller, Luzy Bähr, Silke Feil, Fatma Kivrak Pfiffner, James V. M. Hanson, Alessandro Maspoli, Christina Gerth-Kahlert, Wolfgang Berger
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:International Journal of Molecular Sciences
Subjects:
genetic testing
retinal diseases
sequencing
NGS
diagnostics
long-range PCR
Online Access:https://www.mdpi.com/1422-0067/22/4/1508

Internet

https://www.mdpi.com/1422-0067/22/4/1508

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