Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload
Lead (Pb) poisoning has always been a serious health concern, as it permanently damages the central nervous system. Chronic Pb accumulation in the human body disturbs oligodendrocytes (OLs) differentiation, resulting in dysmyelination, but the molecular mechanism remains unknown. In this study, Pb a...
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doaj-64b83f18650a4c6e8e55fb2aa4ed7c342020-11-24T23:18:56ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-08-01168190961911010.3390/ijms160819096ijms160819096Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium OverloadTeng Ma0Xiyan Wu1Qiyan Cai2Yun Wang3Lan Xiao4Yanping Tian5Hongli Li6Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Histology and Embryology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Histology and Embryology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Histology and Embryology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Histology and Embryology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Histology and Embryology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Histology and Embryology, Third Military Medical University, Chongqing 400038, ChinaLead (Pb) poisoning has always been a serious health concern, as it permanently damages the central nervous system. Chronic Pb accumulation in the human body disturbs oligodendrocytes (OLs) differentiation, resulting in dysmyelination, but the molecular mechanism remains unknown. In this study, Pb at 1 μM inhibits OLs precursor cells (OPCs) differentiation via decreasing the expression of Olig 2, CNPase proteins in vitro. Moreover, Pb treatment inhibits the sodium/calcium exchanger 3 (NCX3) mRNA expression, one of the major means of calcium (Ca2+) extrusion at the plasma membrane during OPCs differentiation. Also addition of KB-R7943, NCX3 inhibitor, to simulate Pb toxicity, resulted in decreased myelin basic protein (MBP) expression and cell branching. Ca2+ response trace with Pb and KB-R7943 treatment did not drop down in the same recovery time as the control, which elevated intracellular Ca2+ concentration reducing MBP expression. In contrast, over-expression of NCX3 in Pb exposed OPCs displayed significant increase MBP fluorescence signal in positive regions and CNPase expression, which recovered OPCs differentiation to counterbalance Pb toxicity. In conclusion, Pb exposure disturbs OLs differentiation via affecting the function of NCX3 by inducing intracellular calcium overload.http://www.mdpi.com/1422-0067/16/8/19096Pb exposureoligodendrocytesdifferentiationsodium/calcium exchanger 3calcium signal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Teng Ma Xiyan Wu Qiyan Cai Yun Wang Lan Xiao Yanping Tian Hongli Li |
spellingShingle |
Teng Ma Xiyan Wu Qiyan Cai Yun Wang Lan Xiao Yanping Tian Hongli Li Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload International Journal of Molecular Sciences Pb exposure oligodendrocytes differentiation sodium/calcium exchanger 3 calcium signal |
author_facet |
Teng Ma Xiyan Wu Qiyan Cai Yun Wang Lan Xiao Yanping Tian Hongli Li |
author_sort |
Teng Ma |
title |
Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload |
title_short |
Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload |
title_full |
Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload |
title_fullStr |
Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload |
title_full_unstemmed |
Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload |
title_sort |
lead poisoning disturbs oligodendrocytes differentiation involved in decreased expression of ncx3 inducing intracellular calcium overload |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-08-01 |
description |
Lead (Pb) poisoning has always been a serious health concern, as it permanently damages the central nervous system. Chronic Pb accumulation in the human body disturbs oligodendrocytes (OLs) differentiation, resulting in dysmyelination, but the molecular mechanism remains unknown. In this study, Pb at 1 μM inhibits OLs precursor cells (OPCs) differentiation via decreasing the expression of Olig 2, CNPase proteins in vitro. Moreover, Pb treatment inhibits the sodium/calcium exchanger 3 (NCX3) mRNA expression, one of the major means of calcium (Ca2+) extrusion at the plasma membrane during OPCs differentiation. Also addition of KB-R7943, NCX3 inhibitor, to simulate Pb toxicity, resulted in decreased myelin basic protein (MBP) expression and cell branching. Ca2+ response trace with Pb and KB-R7943 treatment did not drop down in the same recovery time as the control, which elevated intracellular Ca2+ concentration reducing MBP expression. In contrast, over-expression of NCX3 in Pb exposed OPCs displayed significant increase MBP fluorescence signal in positive regions and CNPase expression, which recovered OPCs differentiation to counterbalance Pb toxicity. In conclusion, Pb exposure disturbs OLs differentiation via affecting the function of NCX3 by inducing intracellular calcium overload. |
topic |
Pb exposure oligodendrocytes differentiation sodium/calcium exchanger 3 calcium signal |
url |
http://www.mdpi.com/1422-0067/16/8/19096 |
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