Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

Abstract Background Molecular tools for detecting malaria-infected mosquitoes with improved practicality, sensitivity and specificity, and high-throughput are required. A common PCR technique used to detect mosquitoes infected with Plasmodium spp. is a nested PCR assay based on the 18s-rRNA gene. Ho...

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Bibliographic Details
Main Authors: Diego F. Echeverry, Nicholas A. Deason, Victoria Makuru, Jenna Davidson, Honglin Xiao, Julie Niedbalski, Xiaoyu Yu, Jennifer C. Stevenson, Hugo Bugoro, Allan Aparaimo, Hedrick Reuben, Robert Cooper, Thomas R. Burkot, Tanya L. Russell, Frank H. Collins, Neil F. Lobo
Format: Article
Language:English
Published: BMC 2017-05-01
Series:Malaria Journal
Subjects:
Malaria
Plasmodium
Diagnosis
Sporozoite
Anopheles
18s-rRNA
Online Access:http://link.springer.com/article/10.1186/s12936-017-1881-1

Internet

http://link.springer.com/article/10.1186/s12936-017-1881-1

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