Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene
Abstract Background Molecular tools for detecting malaria-infected mosquitoes with improved practicality, sensitivity and specificity, and high-throughput are required. A common PCR technique used to detect mosquitoes infected with Plasmodium spp. is a nested PCR assay based on the 18s-rRNA gene. Ho...
Main Authors: | Diego F. Echeverry, Nicholas A. Deason, Victoria Makuru, Jenna Davidson, Honglin Xiao, Julie Niedbalski, Xiaoyu Yu, Jennifer C. Stevenson, Hugo Bugoro, Allan Aparaimo, Hedrick Reuben, Robert Cooper, Thomas R. Burkot, Tanya L. Russell, Frank H. Collins, Neil F. Lobo |
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Format: | Article |
Language: | English |
Published: |
BMC
2017-05-01
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Series: | Malaria Journal |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12936-017-1881-1 |
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