Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota
Abstract Human microbiota-associated (HMA) mice are an important model to study the relationship between liver diseases and intestinal microbiota. We describe a new method to humanize conventional mice based on bowel cleansing with polyethylene glycol followed by fecal microbiota transplantation (FM...
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2018-05-01
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doaj-650da944d3904ca0b9d84f077e2048502020-12-08T04:54:47ZengNature Publishing GroupScientific Reports2045-23222018-05-01811910.1038/s41598-018-25300-3Transplantation of human microbiota into conventional mice durably reshapes the gut microbiotaLaura Wrzosek0Dragos Ciocan1Patrick Borentain2Madeleine Spatz3Virginie Puchois4Cindy Hugot5Gladys Ferrere6Camille Mayeur7Gabriel Perlemuter8Anne-Marie Cassard9INSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINRA, UMR 1319 MICALIS, AgroParisTechINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayINSERM U996, Inflammation Chemokines and Immunopathology, Faculté de Médecine-Univ Paris-Sud, Université Paris-SaclayAbstract Human microbiota-associated (HMA) mice are an important model to study the relationship between liver diseases and intestinal microbiota. We describe a new method to humanize conventional mice based on bowel cleansing with polyethylene glycol followed by fecal microbiota transplantation (FMT) from a human donor. Four successive bowel cleansings were sufficient to empty the intestine and decrease the microbiota by 90%. We then compared four different strategies based on the frequency of FMT over four weeks: (1) twice a week; (2) once a week; (3) two FMTs; (4) one FMT. We were able to transfer human bacteria to mice, irrespective of the strategy used. We detected human bacteria after four weeks, even if only one FMT was performed, but there was a shift of the microbiota over time. FMT twice a week for four weeks was too frequent and perturbed the stability of the newly formed ecosystem. FMT once a week appears to be the best compromise as it allowed engraftment of Faecalibacterium, and a higher diversity of bacteria belonging to the Bacteroidales order. Our easy to establish HMA mouse model could be used as an alternative to classical HMA mice to study the relationship between the liver and the microbiota.https://doi.org/10.1038/s41598-018-25300-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laura Wrzosek Dragos Ciocan Patrick Borentain Madeleine Spatz Virginie Puchois Cindy Hugot Gladys Ferrere Camille Mayeur Gabriel Perlemuter Anne-Marie Cassard |
spellingShingle |
Laura Wrzosek Dragos Ciocan Patrick Borentain Madeleine Spatz Virginie Puchois Cindy Hugot Gladys Ferrere Camille Mayeur Gabriel Perlemuter Anne-Marie Cassard Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota Scientific Reports |
author_facet |
Laura Wrzosek Dragos Ciocan Patrick Borentain Madeleine Spatz Virginie Puchois Cindy Hugot Gladys Ferrere Camille Mayeur Gabriel Perlemuter Anne-Marie Cassard |
author_sort |
Laura Wrzosek |
title |
Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota |
title_short |
Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota |
title_full |
Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota |
title_fullStr |
Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota |
title_full_unstemmed |
Transplantation of human microbiota into conventional mice durably reshapes the gut microbiota |
title_sort |
transplantation of human microbiota into conventional mice durably reshapes the gut microbiota |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-05-01 |
description |
Abstract Human microbiota-associated (HMA) mice are an important model to study the relationship between liver diseases and intestinal microbiota. We describe a new method to humanize conventional mice based on bowel cleansing with polyethylene glycol followed by fecal microbiota transplantation (FMT) from a human donor. Four successive bowel cleansings were sufficient to empty the intestine and decrease the microbiota by 90%. We then compared four different strategies based on the frequency of FMT over four weeks: (1) twice a week; (2) once a week; (3) two FMTs; (4) one FMT. We were able to transfer human bacteria to mice, irrespective of the strategy used. We detected human bacteria after four weeks, even if only one FMT was performed, but there was a shift of the microbiota over time. FMT twice a week for four weeks was too frequent and perturbed the stability of the newly formed ecosystem. FMT once a week appears to be the best compromise as it allowed engraftment of Faecalibacterium, and a higher diversity of bacteria belonging to the Bacteroidales order. Our easy to establish HMA mouse model could be used as an alternative to classical HMA mice to study the relationship between the liver and the microbiota. |
url |
https://doi.org/10.1038/s41598-018-25300-3 |
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