Sequential Genome Editing and Induced Excision of the Transgene in N. tabacum BY2 Cells

Sequential Genome Editing and Induced Excision of the Transgene in N. tabacum BY2 Cells

While plant cells in suspension are becoming a popular platform for expressing biotherapeutic proteins, the need to pre-engineer these cells to better comply with their role as host cell lines is emerging. Heterologous DNA and selectable markers are used for transformation and genome editing designa...

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Bibliographic Details
Main Authors: Maor Sheva, Uri Hanania, Tami Ariel, Albina Turbovski, Vishal Kumar Rameshchandra Rathod, Dina Oz, Yoram Tekoah, Yoseph Shaaltiel
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
genome editing
glyco-engineering
N. tabacum BY2 cells
plant glycans
transgene-free
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2020.607174/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2020.607174/full

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