Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution
Abstract Background Mesophilic alkaline serine proteases from various bacteria have been commercially applied in a range of industries owing to their high catalytic efficiency and wide substrate specificity. However, these proteases have an optimal catalytic temperature of approximately 50 °C, and t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-06-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12896-018-0451-0 |