Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Abstract Background Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (P AOX1 ), and the constitutive GAP promoter (P GAP ). Since promoters play a crucial...

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Bibliographic Details
Main Authors: Javier Garrigós-Martínez, Kiira Vuoristo, Miguel Angel Nieto-Taype, Juha Tähtiharju, Jaana Uusitalo, Pauliina Tukiainen, Christian Schmid, Ilya Tolstorukov, Knut Madden, Merja Penttilä, José Luis Montesinos-Seguí, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Microbial Cell Factories
Subjects:
Komagataella phaffii (Pichia pastoris)
Recombinant protein production
Methanol-free bioprocesses
Expression system characterisation
Promoter regulation
Bioprocess development
Online Access:https://doi.org/10.1186/s12934-021-01564-9

Internet

https://doi.org/10.1186/s12934-021-01564-9

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