Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8
The involvement of spinal release of histamine in the nociceptive behaviors induced by cholecystokinin-8 (CCK-8) was investigated in mice. Intrathecal (i.t.) injection of CCK-8 elicited the nociceptive behaviors consisting of biting and licking. The nociceptive behaviors induced by i.t. treatment wi...
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doaj-663efd92938249f5a9a643304762d46a2020-11-25T02:35:45ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-10-011110.3389/fphar.2020.590918590918Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8Takafumi Hayashi0Chizuko Watanabe1Soh Katsuyama2Yasuyuki Agatsuma3Damiana Scuteri4Giacinto Bagetta5Tsukasa Sakurada6Shinobu Sakurada7Laboratory of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai, JapanDepartment of Physiology and Anatomy, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai, JapanCenter for Clinical Pharmacology and Pharmaceutics, Nihon Pharmaceutical University, Saitama, JapanLaboratory of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai, JapanPreclinical and Translational Pharmacology, Department of Pharmacy, Health Science and Nutrition, University of Calabria, Cosenza, ItalyPreclinical and Translational Pharmacology, Department of Pharmacy, Health Science and Nutrition, University of Calabria, Cosenza, ItalyCenter for Supporting Pharmaceutical Education, Faculty of Pharmaceutical sciences, Daiichi University of Pharmacy, Fukuoka, JapanDepartment of Physiology and Anatomy, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai, JapanThe involvement of spinal release of histamine in the nociceptive behaviors induced by cholecystokinin-8 (CCK-8) was investigated in mice. Intrathecal (i.t.) injection of CCK-8 elicited the nociceptive behaviors consisting of biting and licking. The nociceptive behaviors induced by i.t. treatment with CCK-8 showed two bell-shaped patterns. The histamine H3 receptor antagonist significantly promoted the nociceptive behaviors induced by CCK-8 at doses of 1–100 fmol and 100 pmol. The nociceptive behaviors elicited by CCK-8 was inhibited by i.t. administration of the CCK-B receptor antagonist in a dose-dependent manner, but not by the CCK-A receptor antagonist. The nociceptive behaviors induced by CCK-8 were markedly suppressed by i.t. pretreatment with antiserum against histamine and were abolished in histidine decarboxylase-deleted gene mice. In histamine H1 receptor-deleted gene mice, the nociceptive behaviors induced at both 10 amol and 10 pmol of CCK-8 were not affected. The tachykinin neurokinin-1 (NK1) receptor antagonists inhibited CCK-8 (10 pmol)-induced nociceptive behaviors in a dose-dependent manner. CCK-8 (10 amol)-induced nociceptive behaviors was not antagonized by co-administration with the tachykinin NK1 receptor antagonists. The nociceptive behaviors elicited by CCK-8 were inhibited by i.t. administration of the antagonist for the N-methyl-D-aspartate (NMDA) receptor in a dose-dependent manner. Our results suggest that the nociceptive behaviors induced by i.t. administration of CCK-8 (10 pmol) are mediated through the spinal release of histamine and are elicited via activation of the tachykinin NK1 and NMDA receptors, whereas the nociceptive behaviors induced by i.t. administration of CCK-8 (10 amol) are mediated through the spinal release of histamine and elicited via NMDA receptor activation.https://www.frontiersin.org/articles/10.3389/fphar.2020.590918/fullcholecystokinin-8nociceptive behaviorshistaminespinal cordtachykinin neurokinin-1 receptorN-methyl-D-aspartate receptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takafumi Hayashi Chizuko Watanabe Soh Katsuyama Yasuyuki Agatsuma Damiana Scuteri Giacinto Bagetta Tsukasa Sakurada Shinobu Sakurada |
spellingShingle |
Takafumi Hayashi Chizuko Watanabe Soh Katsuyama Yasuyuki Agatsuma Damiana Scuteri Giacinto Bagetta Tsukasa Sakurada Shinobu Sakurada Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8 Frontiers in Pharmacology cholecystokinin-8 nociceptive behaviors histamine spinal cord tachykinin neurokinin-1 receptor N-methyl-D-aspartate receptor |
author_facet |
Takafumi Hayashi Chizuko Watanabe Soh Katsuyama Yasuyuki Agatsuma Damiana Scuteri Giacinto Bagetta Tsukasa Sakurada Shinobu Sakurada |
author_sort |
Takafumi Hayashi |
title |
Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8 |
title_short |
Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8 |
title_full |
Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8 |
title_fullStr |
Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8 |
title_full_unstemmed |
Contribution of Histamine to Nociceptive Behaviors Induced by Intrathecally Administered Cholecystokinin-8 |
title_sort |
contribution of histamine to nociceptive behaviors induced by intrathecally administered cholecystokinin-8 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2020-10-01 |
description |
The involvement of spinal release of histamine in the nociceptive behaviors induced by cholecystokinin-8 (CCK-8) was investigated in mice. Intrathecal (i.t.) injection of CCK-8 elicited the nociceptive behaviors consisting of biting and licking. The nociceptive behaviors induced by i.t. treatment with CCK-8 showed two bell-shaped patterns. The histamine H3 receptor antagonist significantly promoted the nociceptive behaviors induced by CCK-8 at doses of 1–100 fmol and 100 pmol. The nociceptive behaviors elicited by CCK-8 was inhibited by i.t. administration of the CCK-B receptor antagonist in a dose-dependent manner, but not by the CCK-A receptor antagonist. The nociceptive behaviors induced by CCK-8 were markedly suppressed by i.t. pretreatment with antiserum against histamine and were abolished in histidine decarboxylase-deleted gene mice. In histamine H1 receptor-deleted gene mice, the nociceptive behaviors induced at both 10 amol and 10 pmol of CCK-8 were not affected. The tachykinin neurokinin-1 (NK1) receptor antagonists inhibited CCK-8 (10 pmol)-induced nociceptive behaviors in a dose-dependent manner. CCK-8 (10 amol)-induced nociceptive behaviors was not antagonized by co-administration with the tachykinin NK1 receptor antagonists. The nociceptive behaviors elicited by CCK-8 were inhibited by i.t. administration of the antagonist for the N-methyl-D-aspartate (NMDA) receptor in a dose-dependent manner. Our results suggest that the nociceptive behaviors induced by i.t. administration of CCK-8 (10 pmol) are mediated through the spinal release of histamine and are elicited via activation of the tachykinin NK1 and NMDA receptors, whereas the nociceptive behaviors induced by i.t. administration of CCK-8 (10 amol) are mediated through the spinal release of histamine and elicited via NMDA receptor activation. |
topic |
cholecystokinin-8 nociceptive behaviors histamine spinal cord tachykinin neurokinin-1 receptor N-methyl-D-aspartate receptor |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2020.590918/full |
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