Monitoring protein phosphorylation by acrylamide pendant Phos-Tag™ in various plants

Monitoring protein phosphorylation by acrylamide pendant Phos-Tag™ in various plants

The aim of the present study is to rationalize acrylamide pendant Phos-Tag™ in-gel discrimination of phosphorylated and non-phosphorylated plant protein species with standard immunoblot analysis, and optimize sample preparation, efficient electrophoretic separation and transfer. We tested variants o...

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Bibliographic Details
Main Authors: Slavka eBekesova, George eKomis, Pavel eKrenek, Petra eVyplelova, Miroslav eOvecka, Ivan eLuptovciak, Peter eIlles, Anna eKucharova, Jozef eSamaj
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-05-01
Series:Frontiers in Plant Science
Subjects:
Medicago sativa
Arabidopsis thaliana
Protein phosphorylation
Hordeum vulgare
mitogen activated protein kinase
Triticum turgidum
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00336/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00336/full

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