Comparative analysis of sequencing technologies for single-cell transcriptomics

Comparative analysis of sequencing technologies for single-cell transcriptomics

Abstract Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, perfo...

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Bibliographic Details
Main Authors: Kedar Nath Natarajan, Zhichao Miao, Miaomiao Jiang, Xiaoyun Huang, Hongpo Zhou, Jiarui Xie, Chunqing Wang, Shishang Qin, Zhikun Zhao, Liang Wu, Naibo Yang, Bo Li, Yong Hou, Shiping Liu, Sarah A. Teichmann
Format: Article
Language:English
Published: BMC 2019-04-01
Series:Genome Biology
Subjects:
Single-cell RNA sequencing
Sequencing platforms
Benchmarking scRNA-seq
Illumina sequencing
BGISEQ-500
Online Access:http://link.springer.com/article/10.1186/s13059-019-1676-5

Internet

http://link.springer.com/article/10.1186/s13059-019-1676-5

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