The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase

The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase

Single-particle electron cryo-microscopy (cryoEM) has undergone a `resolution revolution' that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expr...

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Bibliographic Details
Main Authors: Mirko Joppe, Edoardo D'Imprima, Nina Salustros, Karthik S. Paithankar, Janet Vonck, Martin Grininger, Werner Kühlbrandt
Format: Article
Language:English
Published: International Union of Crystallography 2020-03-01
Series:IUCrJ
Subjects:
purification of protein complexes
3d reconstruction and image processing
single-particle cryoem
cryo-electron microscopy
macromolecular machines
protein structure
yeast fatty acid synthase
Online Access:http://scripts.iucr.org/cgi-bin/paper?S2052252519017366

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http://scripts.iucr.org/cgi-bin/paper?S2052252519017366

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