Production and Characterization of Recombinant Wild Type Uricase from Indonesian Coelacanth (L. menadoensis) and Improvement of Its Thermostability by In Silico Rational Design and Disulphide Bridges Engineering

Production and Characterization of Recombinant Wild Type Uricase from Indonesian Coelacanth (L. menadoensis) and Improvement of Its Thermostability by In Silico Rational Design and Disulphide Bridges Engineering

The ideal therapeutic uricase (UOX) is expected to have the following properties; high expression level, high activity, high thermostability, high solubility and low immunogenicity. The latter property is believed to depend largely on sequence identity to the deduced human UOX (dH-UOX). Herein, we e...

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Bibliographic Details
Main Authors: Sakda Yainoy, Thanawat Phuadraksa, Sineewanlaya Wichit, Maprang Sompoppokakul, Napat Songtawee, Virapong Prachayasittikul, Chartchalerm Isarankura-Na-Ayudhya
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:International Journal of Molecular Sciences
Subjects:
uricase
coelacanth
Latimeria
disulphide bond engineering
hyperuricemia
Online Access:http://www.mdpi.com/1422-0067/20/6/1269

Internet

http://www.mdpi.com/1422-0067/20/6/1269

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