The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression.
BACKGROUND:Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS:We provide new evidence showing that overexpression of h...
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doaj-67bb874c3bd942d0bac7797164de3c092020-11-25T02:23:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2179610.1371/journal.pone.0021796The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression.Sung-Young LeeJaeho YoonHyun-Shik LeeYoo-Seok HwangSang-Wook ChaChul-Ho JeongJong-Il KimJae-Bong ParkJae-Yong LeeSungChan KimMae Ja ParkZigang DongJaebong KimBACKGROUND:Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS:We provide new evidence showing that overexpression of heterodimeric AP-1 comprised of c-jun and c-fos (AP-1(c-Jun/c-Fos)) induces the expression of BMP-antagonizing organizer genes (noggin, chordin and goosecoid) that were normally expressed by high dose of activin. AP-1(c-Jun/c-Fos) enhanced the promoter activities of organizer genes but reduced that of PV.1, a BMP4-response gene. A loss of function study clearly demonstrated that AP-1(c-Jun/c-Fos) is required for the activin-induced organizer and neural gene expression. Moreover, physical interaction of AP-1(c-Jun/c-Fos) and Smad3 cooperatively enhanced the transcriptional activity of goosecoid via direct binding on this promoter. Interestingly, Smad3 mutants at c-Jun binding site failed in regulation of organizer genes, indicating that these physical interactions are specifically necessary for the expression of organizer genes. CONCLUSIONS/SIGNIFICANCE:AP-1(c-Jun/c-Fos) plays a specific role in organizer gene expression in downstream of activin signal during early Xenopus embryogenesis.http://europepmc.org/articles/PMC3146467?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sung-Young Lee Jaeho Yoon Hyun-Shik Lee Yoo-Seok Hwang Sang-Wook Cha Chul-Ho Jeong Jong-Il Kim Jae-Bong Park Jae-Yong Lee SungChan Kim Mae Ja Park Zigang Dong Jaebong Kim |
spellingShingle |
Sung-Young Lee Jaeho Yoon Hyun-Shik Lee Yoo-Seok Hwang Sang-Wook Cha Chul-Ho Jeong Jong-Il Kim Jae-Bong Park Jae-Yong Lee SungChan Kim Mae Ja Park Zigang Dong Jaebong Kim The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. PLoS ONE |
author_facet |
Sung-Young Lee Jaeho Yoon Hyun-Shik Lee Yoo-Seok Hwang Sang-Wook Cha Chul-Ho Jeong Jong-Il Kim Jae-Bong Park Jae-Yong Lee SungChan Kim Mae Ja Park Zigang Dong Jaebong Kim |
author_sort |
Sung-Young Lee |
title |
The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
title_short |
The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
title_full |
The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
title_fullStr |
The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
title_full_unstemmed |
The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
title_sort |
function of heterodimeric ap-1 comprised of c-jun and c-fos in activin mediated spemann organizer gene expression. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
BACKGROUND:Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS:We provide new evidence showing that overexpression of heterodimeric AP-1 comprised of c-jun and c-fos (AP-1(c-Jun/c-Fos)) induces the expression of BMP-antagonizing organizer genes (noggin, chordin and goosecoid) that were normally expressed by high dose of activin. AP-1(c-Jun/c-Fos) enhanced the promoter activities of organizer genes but reduced that of PV.1, a BMP4-response gene. A loss of function study clearly demonstrated that AP-1(c-Jun/c-Fos) is required for the activin-induced organizer and neural gene expression. Moreover, physical interaction of AP-1(c-Jun/c-Fos) and Smad3 cooperatively enhanced the transcriptional activity of goosecoid via direct binding on this promoter. Interestingly, Smad3 mutants at c-Jun binding site failed in regulation of organizer genes, indicating that these physical interactions are specifically necessary for the expression of organizer genes. CONCLUSIONS/SIGNIFICANCE:AP-1(c-Jun/c-Fos) plays a specific role in organizer gene expression in downstream of activin signal during early Xenopus embryogenesis. |
url |
http://europepmc.org/articles/PMC3146467?pdf=render |
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