Efficient Knockdown and Lack of Passenger Strand Activity by Dicer-Independent shRNAs Expressed from Pol II-Driven MicroRNA Scaffolds

Efficient Knockdown and Lack of Passenger Strand Activity by Dicer-Independent shRNAs Expressed from Pol II-Driven MicroRNA Scaffolds

The expression of short hairpin RNAs (shRNAs) may result in unwanted activity from the co-processed passenger strand. Recent studies have shown that shortening the stem of conventional shRNAs abolishes passenger strand release. These Dicer-independent shRNAs, expressed from RNA polymerase III (Pol I...

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Bibliographic Details
Main Authors: Erik Kaadt, Sidsel Alsing, Claudia R. Cecchi, Christian Kroun Damgaard, Thomas J. Corydon, Lars Aagaard
Format: Article
Language:English
Published: Elsevier 2019-03-01
Series:Molecular Therapy: Nucleic Acids
Online Access:http://www.sciencedirect.com/science/article/pii/S216225311830307X

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http://www.sciencedirect.com/science/article/pii/S216225311830307X

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