Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and develop a model which ranks off-target sites.

Bibliographic Details
Main Authors: Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu
Format: Article
Language:English
Published: Nature Publishing Group 2018-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-05391-2

Internet

https://doi.org/10.1038/s41467-018-05391-2

Similar Items