Nondenaturing purification of co-transcriptionally folded RNA avoids common folding heterogeneity.

Nondenaturing purification of co-transcriptionally folded RNA avoids common folding heterogeneity.

Due to the energetic frustration of RNA folding, tertiary structured RNA is typically characterized by a rugged folding free energy landscape where deep kinetic barriers separate numerous misfolded states from one or more native states. While most in vitro studies of RNA rely on (re)folding chemical...

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Bibliographic Details
Main Authors: Miguel J B Pereira, Vivek Behera, Nils G Walter
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-09-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2944885?pdf=render

Internet

http://europepmc.org/articles/PMC2944885?pdf=render

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