HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300
Abstract Background HIV-associated neurocognitive disorder (HAND) is a neurodegenerative disease associated with persistent neuroinflammation and subsequent neuron damage. Pro-inflammatory factors and neurotoxins from activated astrocytes by HIV-1 itself and its encoded proteins, including the negat...
Main Authors: | , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2018-10-01
|
Series: | Journal of Neuroinflammation |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12974-018-1343-x |
id |
doaj-689dc8834ce64ebc8cff47e1f2748b5f |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Zhou Xiaomei Liu Dongjiao Zuo Min Xue Lin Gao Ying Yang Jing Wang Liping Niu Qianwen Cao Xiangyang Li Hui Hua Bo Zhang Minmin Hu Dianshuai Gao Kuiyang Zheng Yoshihiro Izumiya Renxian Tang |
spellingShingle |
Feng Zhou Xiaomei Liu Dongjiao Zuo Min Xue Lin Gao Ying Yang Jing Wang Liping Niu Qianwen Cao Xiangyang Li Hui Hua Bo Zhang Minmin Hu Dianshuai Gao Kuiyang Zheng Yoshihiro Izumiya Renxian Tang HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300 Journal of Neuroinflammation HIV-associated neurocognitive disorder HIV-1 lncRNAs Astrocytes Nef CBP/P300 |
author_facet |
Feng Zhou Xiaomei Liu Dongjiao Zuo Min Xue Lin Gao Ying Yang Jing Wang Liping Niu Qianwen Cao Xiangyang Li Hui Hua Bo Zhang Minmin Hu Dianshuai Gao Kuiyang Zheng Yoshihiro Izumiya Renxian Tang |
author_sort |
Feng Zhou |
title |
HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300 |
title_short |
HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300 |
title_full |
HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300 |
title_fullStr |
HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300 |
title_full_unstemmed |
HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300 |
title_sort |
hiv-1 nef-induced lncrna ak006025 regulates cxcl9/10/11 cluster gene expression in astrocytes through interaction with cbp/p300 |
publisher |
BMC |
series |
Journal of Neuroinflammation |
issn |
1742-2094 |
publishDate |
2018-10-01 |
description |
Abstract Background HIV-associated neurocognitive disorder (HAND) is a neurodegenerative disease associated with persistent neuroinflammation and subsequent neuron damage. Pro-inflammatory factors and neurotoxins from activated astrocytes by HIV-1 itself and its encoded proteins, including the negative factor (Nef), are involved in the pathogenesis of HAND. This study was designed to find potential lncRNAs that regulate astrocyte functions and inflammation process. Methods We performed microarray analysis of lncRNAs from primary mouse astrocytes treated with Nef protein. Top ten lncRNAs were validated through real-time PCR analysis. Gene ontology (GO) and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. RIP and ChIP assays were performed to demonstrate the mechanism of lncRNA regulating gene expression. Results There were 638 co-upregulated lncRNAs and 372 co-downregulated lncRNAs in primary astrocytes treated with Nef protein for both 6 h and 12 h. GO and KEGG pathway analysis showed that the biological functions of top differential-expressed mRNAs were associated with inflammatory cytokines and chemokine. Knockdown of lncRNA AK006025, not AK138360, inhibited significantly CXCL9, CXCL10 (IP-10), and CXCL11 expression in astrocytes treated with Nef protein. Mechanism study showed that AK006025 associated with CBP/P300 was enriched in the promoter of CXCL9, CXCL10, and CXCL11 genes. Conclusions Our findings uncovered the expression profiles of lncRNAs and mRNAs in vitro, which might help to understand the pathways that regulate astrocyte activation during the process of HAND. |
topic |
HIV-associated neurocognitive disorder HIV-1 lncRNAs Astrocytes Nef CBP/P300 |
url |
http://link.springer.com/article/10.1186/s12974-018-1343-x |
work_keys_str_mv |
AT fengzhou hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT xiaomeiliu hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT dongjiaozuo hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT minxue hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT lingao hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT yingyang hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT jingwang hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT lipingniu hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT qianwencao hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT xiangyangli hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT huihua hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT bozhang hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT minminhu hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT dianshuaigao hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT kuiyangzheng hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT yoshihiroizumiya hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 AT renxiantang hiv1nefinducedlncrnaak006025regulatescxcl91011clustergeneexpressioninastrocytesthroughinteractionwithcbpp300 |
_version_ |
1724948948247904256 |
spelling |
doaj-689dc8834ce64ebc8cff47e1f2748b5f2020-11-25T02:03:11ZengBMCJournal of Neuroinflammation1742-20942018-10-0115111410.1186/s12974-018-1343-xHIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300Feng Zhou0Xiaomei Liu1Dongjiao Zuo2Min Xue3Lin Gao4Ying Yang5Jing Wang6Liping Niu7Qianwen Cao8Xiangyang Li9Hui Hua10Bo Zhang11Minmin Hu12Dianshuai Gao13Kuiyang Zheng14Yoshihiro Izumiya15Renxian Tang16Jiangsu Key Laboratory of Brain Disease Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityDepartment of Physiology, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityDepartment of Neurobiology and Anatomy, Xuzhou Medical UniversityJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityDepartment of Dermatology, University of California Davis (UC Davis) School of MedicineJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology and Laboratory of Infection and Immunity, Xuzhou Medical UniversityAbstract Background HIV-associated neurocognitive disorder (HAND) is a neurodegenerative disease associated with persistent neuroinflammation and subsequent neuron damage. Pro-inflammatory factors and neurotoxins from activated astrocytes by HIV-1 itself and its encoded proteins, including the negative factor (Nef), are involved in the pathogenesis of HAND. This study was designed to find potential lncRNAs that regulate astrocyte functions and inflammation process. Methods We performed microarray analysis of lncRNAs from primary mouse astrocytes treated with Nef protein. Top ten lncRNAs were validated through real-time PCR analysis. Gene ontology (GO) and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. RIP and ChIP assays were performed to demonstrate the mechanism of lncRNA regulating gene expression. Results There were 638 co-upregulated lncRNAs and 372 co-downregulated lncRNAs in primary astrocytes treated with Nef protein for both 6 h and 12 h. GO and KEGG pathway analysis showed that the biological functions of top differential-expressed mRNAs were associated with inflammatory cytokines and chemokine. Knockdown of lncRNA AK006025, not AK138360, inhibited significantly CXCL9, CXCL10 (IP-10), and CXCL11 expression in astrocytes treated with Nef protein. Mechanism study showed that AK006025 associated with CBP/P300 was enriched in the promoter of CXCL9, CXCL10, and CXCL11 genes. Conclusions Our findings uncovered the expression profiles of lncRNAs and mRNAs in vitro, which might help to understand the pathways that regulate astrocyte activation during the process of HAND.http://link.springer.com/article/10.1186/s12974-018-1343-xHIV-associated neurocognitive disorderHIV-1lncRNAsAstrocytesNefCBP/P300 |