ARDEP, a Rapid Degenerate Primer Design Pipeline Based on <i>k</i>-mers for Amplicon Microbiome Studies

The survey of microbial diversity in various environments has relied upon the widespread use of well-evaluated amplification primers for taxonomic marker genes (e.g., prokaryotic 16S and fungal ITS). However, it is urgent to develop a fast and accurate bioinformatic program to design primers for mic...

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Bibliographic Details
Main Authors: Yueni Wu, Kai Feng, Ziyan Wei, Zhujun Wang, Ye Deng
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:International Journal of Environmental Research and Public Health
Subjects:
Online Access:https://www.mdpi.com/1660-4601/17/16/5958