The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2
To investigate the potential role of nuclear factor erythroid 2-related factor 2 (Nrf2) in licorice ethanol extract (LEE) against triptolide- (TP-) induced hepatotoxicity, HepG2 cells were exposed to LEE (30, 60, and 90 mg·L−1) for 12 h and then treated with TP (50 nM) for 24 h. Besides, an acute li...
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doaj-69348f1e33c545c8bf5e1e468b1885cd2020-11-24T21:24:17ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882017-01-01201710.1155/2017/27523892752389The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2Ling-Juan Cao0Zhen-Yan Hou1Huan-De Li2Bi-Kui Zhang3Ping-Fei Fang4Da-Xiong Xiang5Zhi-Hua Li6Hui Gong7Yang Deng8Yan-Xia Ma9Huai-Bo Tang10Miao Yan11Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaDepartment of Pharmacy, Chemistry College, Xiangtan University, Xiangtan 411105, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha 410011, ChinaTo investigate the potential role of nuclear factor erythroid 2-related factor 2 (Nrf2) in licorice ethanol extract (LEE) against triptolide- (TP-) induced hepatotoxicity, HepG2 cells were exposed to LEE (30, 60, and 90 mg·L−1) for 12 h and then treated with TP (50 nM) for 24 h. Besides, an acute liver injury model was established in ICR mice by a single dose of TP (1.0 mg·kg−1, i.p.). Relevant oxidant and antioxidant mediators were analyzed. TP led to an obvious oxidative stress as evidenced by increasing levels of ROS and decreasing GSH contents in HepG2 cells. In vitro results were likely to hold true in in vivo experiments. LEE protected against TP-induced oxidative stress in both in vitro and in vivo conditions. Furthermore, the decreased level of Nrf2 in the TP-treated group was observed. The mRNA levels of downstream genes decreased as well in ICR mice liver, whereas they increased in HepG2 cells. In contrast, LEE pretreatment significantly increased the level of Nrf2 and its downstream genes. LEE protects against TP-induced oxidative stress partly via the activation of Nrf2 pathway.http://dx.doi.org/10.1155/2017/2752389 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ling-Juan Cao Zhen-Yan Hou Huan-De Li Bi-Kui Zhang Ping-Fei Fang Da-Xiong Xiang Zhi-Hua Li Hui Gong Yang Deng Yan-Xia Ma Huai-Bo Tang Miao Yan |
spellingShingle |
Ling-Juan Cao Zhen-Yan Hou Huan-De Li Bi-Kui Zhang Ping-Fei Fang Da-Xiong Xiang Zhi-Hua Li Hui Gong Yang Deng Yan-Xia Ma Huai-Bo Tang Miao Yan The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2 Evidence-Based Complementary and Alternative Medicine |
author_facet |
Ling-Juan Cao Zhen-Yan Hou Huan-De Li Bi-Kui Zhang Ping-Fei Fang Da-Xiong Xiang Zhi-Hua Li Hui Gong Yang Deng Yan-Xia Ma Huai-Bo Tang Miao Yan |
author_sort |
Ling-Juan Cao |
title |
The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2 |
title_short |
The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2 |
title_full |
The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2 |
title_fullStr |
The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2 |
title_full_unstemmed |
The Ethanol Extract of Licorice (Glycyrrhiza uralensis) Protects against Triptolide-Induced Oxidative Stress through Activation of Nrf2 |
title_sort |
ethanol extract of licorice (glycyrrhiza uralensis) protects against triptolide-induced oxidative stress through activation of nrf2 |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2017-01-01 |
description |
To investigate the potential role of nuclear factor erythroid 2-related factor 2 (Nrf2) in licorice ethanol extract (LEE) against triptolide- (TP-) induced hepatotoxicity, HepG2 cells were exposed to LEE (30, 60, and 90 mg·L−1) for 12 h and then treated with TP (50 nM) for 24 h. Besides, an acute liver injury model was established in ICR mice by a single dose of TP (1.0 mg·kg−1, i.p.). Relevant oxidant and antioxidant mediators were analyzed. TP led to an obvious oxidative stress as evidenced by increasing levels of ROS and decreasing GSH contents in HepG2 cells. In vitro results were likely to hold true in in vivo experiments. LEE protected against TP-induced oxidative stress in both in vitro and in vivo conditions. Furthermore, the decreased level of Nrf2 in the TP-treated group was observed. The mRNA levels of downstream genes decreased as well in ICR mice liver, whereas they increased in HepG2 cells. In contrast, LEE pretreatment significantly increased the level of Nrf2 and its downstream genes. LEE protects against TP-induced oxidative stress partly via the activation of Nrf2 pathway. |
url |
http://dx.doi.org/10.1155/2017/2752389 |
work_keys_str_mv |
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