Liquid Chromatography with Tandem Mass Spectrometry: A Sensitive Method for the Determination of Dehydrodiisoeugenol in Rat Cerebral Nuclei

• Main Authors: You-Bo Zhang, Xin-Bao Yang, Xiu-Wei Yang, Wei Xu, Fei Li, Frank J. Gonzezal
• Format: Article
• Language: English
• Published: MDPI AG 2016-03-01
• Series: Molecules
• Subjects: HPLC-MS/MS; cerebral nuclei; drug distribution; nutmeg; Myristica fragrans; dehydrodiisoeugenol
• Online Access: http://www.mdpi.com/1420-3049/21/3/321

A new liquid chromatography–tandem mass spectrometry (LC-MS/MS) method is developed for the quantification of dehydrodiisoeugenol (DDIE) in rat cerebral nuclei after single intravenous administration. DDIE and daidzein (internal standard) were separated on a Diamonsil™ ODS C18 column with methanol–water containing 0.1% formic acid (81:19, v/v) as a mobile phase. Detection of DDIE was performed on a positive electrospray ionization source using a triple quadrupole mass spectrometer. DDIE and daidzein were monitored at m/z 327.2→188.0 and m/z 255.0→199.2, respectively, in multiple reaction monitoring mode. This method enabled quantification of DDIE in various brain areas, including, cortex, hippocampus, striatum, hypothalamus, cerebellum and brainstem, with high specificity, precision, accuracy, and recovery. The data herein demonstrate that our new LC-MS/MS method is highly sensitive and suitable for monitoring cerebral nuclei distribution of DDIE.