Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples.

OBJECTIVES:There is much speculation on which hypervariable region provides the highest bacterial specificity in 16S rRNA sequencing. The optimum solution to prevent bias and to obtain a comprehensive view of complex bacterial communities would be to sequence the entire 16S rRNA gene; however, this...

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Bibliographic Details
Main Authors: Jennifer J Barb, Andrew J Oler, Hyung-Suk Kim, Natalia Chalmers, Gwenyth R Wallen, Ann Cashion, Peter J Munson, Nancy J Ames
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4734828?pdf=render