Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples.

Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples.

OBJECTIVES:There is much speculation on which hypervariable region provides the highest bacterial specificity in 16S rRNA sequencing. The optimum solution to prevent bias and to obtain a comprehensive view of complex bacterial communities would be to sequence the entire 16S rRNA gene; however, this...

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Bibliographic Details
Main Authors: Jennifer J Barb, Andrew J Oler, Hyung-Suk Kim, Natalia Chalmers, Gwenyth R Wallen, Ann Cashion, Peter J Munson, Nancy J Ames
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4734828?pdf=render

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http://europepmc.org/articles/PMC4734828?pdf=render

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