Optimization and clinical validation of a Real-Time PCR protocol for direct detection of Trichomonas vaginalis in pooled urine samples
Background and Objectives: A new Real- Time PCR protocol for the detection of Trichomonas vaginalis in pooled urine samples has been optimized and validated. Materials and Methods: The amplification protocol, targeting a 2kb repeated gene in the T. vaginalis genome, was optimized by varying PCR par...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2009-09-01
|
Series: | Iranian Journal of Microbiology |
Subjects: | |
Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/23 |