Optimization and clinical validation of a Real-Time PCR protocol for direct detection of Trichomonas vaginalis in pooled urine samples

Background and Objectives: A new Real- Time PCR protocol for the detection of Trichomonas vaginalis in pooled urine samples has been optimized and validated. Materials and Methods: The amplification protocol, targeting a 2kb repeated gene in the T. vaginalis genome, was optimized by varying PCR par...

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Bibliographic Details
Main Authors: WB van Leeuwen, WHA Zandijk
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2009-09-01
Series:Iranian Journal of Microbiology
Subjects:
Online Access:https://ijm.tums.ac.ir/index.php/ijm/article/view/23