Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region

Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region

We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardiand Mansonella perstans) that can be found in the Amazon Region. Nested PCR-bas...

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Bibliographic Details
Main Authors: Thuy-Huong Ta Tang, Rogelio López-Vélez, Marta Lanza, Anthony John Shelley, Jose Miguel Rubio, Sérgio Luiz Bessa Luz
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2010-09-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
nested PCR
18S rDNA
ITS-1
onchocerciasis
mansonelliasis
Brazil
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600016

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600016

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